Long-read 16S rRNA Gene Sequencing and Analysis Service

Gain deeper insights into microbial communities when you take advantage of Loop's highly accurate single molecule counting and long-read Long-read 16S rRNA gene sequencing service.

LONG-READ SEQUENCING AT SHORT READ PRICES

What you get with Loop's Long-read 16S rRNA Gene Sequencing Service 

  • Sequence of the full-length 16S​ rRNA gene

  • >99% of unique 16S molecules assigned at the species or genus level

  • Accurate organism abundance measurement

  • 40x lower error rates than Illumina or PacBio platforms

  • Easy-to-understand reports and raw data

  • Highly affordable high volume pricing

    • $79/sample for 96 samples​

    • $69/sample for 288 samples​

    • $59/sample for 480 samples​

    • $49/sample for 672 samples​

    • $35/sample for 960 samples

  • Low volume pricing available, minimum 8 samples, inquire for pricing

$79 per sample for 96 samples to $35 per sample for 1,000+ samples

Gain a deeper understanding of the composition of your sample when you sequence the complete 16S rRNA gene.

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Stay focused on biology and leave the sequencing and bioinformatics to Loop!

Reports include csv files, pie charts, plots, and other data visualizations:
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  • Single molecule quantification

  • Reference-based taxonomic classifications

  • Operational taxonomic unit (OTU) tables

  • Rarefaction analysis

  • RAD analysis

  • Alpha diversity analysis (Shannon and Simpson)

  • Raw data as FASTA and FASTQ files 

  • See real data in an interactive report for 8 single-species samples from ATCC and for a ZymoBIOMICS Mock Community Standard (note the reproducibility - the same sample is run in 24 wells)

16S rRNA Gene Sequencing and Data Analysis

Service description:

We will use our LoopSeq single molecule-counting long-read 16S Ribosomal DNA kit to sequence your microbiome samples to determine the type and relative abundance of bacterial and archaeal species. We can accept purified DNA from gut, skin, soil, marine or other sources.

Advantages:

  • Higher sensitivity than other 16s services as we interrogate the entire 16s molecule V1-V9  with our single molecule counting long-read technology

  • 40x lower error when compared to Illumina library prep

  • Cost-effective

  • Fast turnaround 

Region Coverage:

Full 16s molecule, all V1-V9 variable regions

Deliverables:

Our standard analysis includes CSV files with (1) Single molecule quantification of abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases, (2) Reference based taxonomic classification based on nine variable regions (V1-V9 data in 16S) and long-reads in Metagenomic sequencing, (3) OTU tables, (4) Rarefaction analysis, (5) RAD analysis, as well as pie chart visualizations of classification and quantification.(5) Raw data as FASTA and FASTQ files.

Turn Around Time: 4 weeks 

Sample submission:

High quality genomic DNA , a minimum of 10ng as quantified by Qubit 2.0, or 1ng DNA from skin

Minimum number of samples: 8 (Learn about high-volume pricing here)

16S/18S rRNA Gene Sequencing and Data Analysis

Service description:

We will use our LoopSeq single molecule-counting long-read 16S/18S Ribosomal DNA kit to sequence your microbiome samples to determine the type and relative abundance of bacterial and archaeal species. We can accept purified DNA from gut, skin, soil, marine or other sources.

Advantages:

  • Higher sensitivity than other 16s/18s services as we interrogate the entire 16s/18s molecule V1-V9  with our single molecule counting long-read technology

  • 40x lower error when compared to Illumina library prep

  • Cost-effective

  • Fast turnaround 

Region coverage:

Full 16s/18s molecules, all V1-V9 variable regions

Deliverables:

Our standard analysis includes CSV files with (1) Single molecule quantification of abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases, (2) Reference based taxonomic classification based on nine variable regions (V1-V9 data in 16S and 18S) and long-reads in Metagenomic sequencing, (3) OTU tables, (4) Rarefaction analysis, (5) RAD analysis, as well as pie chart visualizations of classification and quantification.(5) Raw data as FASTA and FASTQ files.

Turn-around time: 3 weeks 

Sample submission:

High quality genomic DNA , a minimum of 10ng as quantified by Qubit 2.0, or 1ng DNA from skin

Minimum Number of Samples: 8

Classify Archaeal Species with our 16S/18S rRNA Gene Sequencing Service