Insight that's affordable 

LoopSeq Long-read Bacterial Whole Genome Sequencing

Improve bacterial genome assembly and sequence accuracy

With LoopSeq's long-read technology, you get all the affordability of Illumina short-read sequencing, the assembly power of a long-read approach, and unparalleled sequence accuracy that's unique to Loop Genomics. For bacterial genomes, these advantages translate into:

  • Fewer, longer contigs

  • More comprehensive coverage

  • Better quality scores

  • More complete assemblies 

Demonstrably better

Regardless of GC content or genome size, the LoopSeq Genome Kit consistently outperforms conventional short-read approaches.

LoopSeq reads assemble into 89- to 6-fold fewer contigs than PE150 reads or PE300 reads.

LoopSeq assemblies average a 5.7-fold larger N50 value compared to PE150 assemblies, indicating more comprehensive genome coverage.

The largest contig assembled from LoopSeq reads averages 2.6-fold larger than the largest contig assembled from PE150 or PE300 reads.

LoopSeq assemblies average a 6.2-fold lower LGA50 value compared to PE150 assemblies, indicating more comprehensive genome coverage.

How it works

Input.

Input any linear dsDNA molecule.

Attach.

Every sample is exposed to millions of unique barcodes, but only one barcode attaches per dsDNA molecule

Amplify.

Every molecule, along with its unique barcode, is amplified using PCR.

Distribute.

For each molecule copy, the barcode is randomly distributed within the molecule.

Sequence.

Sequence the segment next to each barcode.

Assemble.

Short reads that share the same barcode are combined algorithmically into a full-length molecule using linked-read de novo assembly.

Benefits.

Multiplex workflow

Pool all samples into a single tube. 

Full-length genes

Single-molecule long-read data.

Reduced Error Rate

Higher resolution sequence data.

UMI based DNA counting

Barcode based quantification with no PCR bias.

Easy to use

Get the kit

Next day shipping for the sample prep kit.

Upload results

Securely upload FASTQ results to the Loop's cloud pipeline.

Receive data

Download FASTQ and CSV files with classification and quantification data.

We've been featured

The exciting thing about [the tech] is that all you need to get started is a sample prep kit.

...better quantify low abundance species than standard Illumina sequencing.

Outsource your sequencing

Process your samples in Loop's lab. Learn more about the service options.

Project highlight

See how Loop helps Whole Biome advance diabetes research.
Loop Genomics
5941 Optical Court
San Jose, CA 95138 USA
Tel: 650-815-7088
Toll-Free: 844-238-1849
Fax: 650-285-2387
sales@loopgenomics.com
techsupport@loopgenomics.com

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