Improve bacterial genome assembly and sequence accuracy
With LoopSeq's long-read technology, you get all the affordability of Illumina short-read sequencing, the assembly power of a long-read approach, and unparalleled sequence accuracy that's unique to Loop Genomics. For bacterial genomes, these advantages translate into:
Fewer, longer contigs
More comprehensive coverage
Better quality scores
More complete assemblies
Regardless of GC content or genome size, the LoopSeq Genome Kit consistently outperforms conventional short-read approaches.
LoopSeq reads assemble into 89- to 6-fold fewer contigs than PE150 reads or PE300 reads.
LoopSeq assemblies average a 5.7-fold larger N50 value compared to PE150 assemblies, indicating more comprehensive genome coverage.
The largest contig assembled from LoopSeq reads averages 2.6-fold larger than the largest contig assembled from PE150 or PE300 reads.
LoopSeq assemblies average a 6.2-fold lower LGA50 value compared to PE150 assemblies, indicating more comprehensive genome coverage.
How it works
Input any linear dsDNA molecule.
Every sample is exposed to millions of unique barcodes, but only one barcode attaches per dsDNA molecule
Every molecule, along with its unique barcode, is amplified using PCR.
For each molecule copy, the barcode is randomly distributed within the molecule.
Sequence the segment next to each barcode.
Short reads that share the same barcode are combined algorithmically into a full-length molecule using linked-read de novo assembly.
Pool all samples into a single tube.
Single-molecule long-read data.
Reduced Error Rate
Higher resolution sequence data.
UMI based DNA counting
Barcode based quantification with no PCR bias.
Easy to use
Get the kit
Next day shipping for the sample prep kit.
Securely upload FASTQ results to the Loop's cloud pipeline.
Download FASTQ and CSV files with classification and quantification data.