Insight that's affordable
LoopSeq Long-read Bacterial Whole Genome Sequencing
Improve bacterial genome assembly and sequence accuracy
With LoopSeq's long-read technology, you get all the affordability of Illumina short-read sequencing, the assembly power of a long-read approach, and unparalleled sequence accuracy that's unique to Loop Genomics. For bacterial genomes, these advantages translate into:
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Fewer, longer contigs
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More comprehensive coverage
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Better quality scores
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More complete assemblies
Demonstrably better
Regardless of GC content or genome size, the LoopSeq Genome Kit consistently outperforms conventional short-read approaches.
LoopSeq reads assemble into 89- to 6-fold fewer contigs than PE150 reads or PE300 reads.
LoopSeq assemblies average a 5.7-fold larger N50 value compared to PE150 assemblies, indicating more comprehensive genome coverage.
The largest contig assembled from LoopSeq reads averages 2.6-fold larger than the largest contig assembled from PE150 or PE300 reads.
LoopSeq assemblies average a 6.2-fold lower LGA50 value compared to PE150 assemblies, indicating more comprehensive genome coverage.
How it works
Input.
Input any linear dsDNA molecule.
Attach.
Every sample is exposed to millions of unique barcodes, but only one barcode attaches per dsDNA molecule
Amplify.
Every molecule, along with its unique barcode, is amplified using PCR.
Distribute.
For each molecule copy, the barcode is randomly distributed within the molecule.
Sequence.
Sequence the segment next to each barcode.
Assemble.
Short reads that share the same barcode are combined algorithmically into a full-length molecule using linked-read de novo assembly.
Benefits.
Multiplex workflow
Pool all samples into a single tube.
Full-length genes
Single-molecule long-read data.
Reduced Error Rate
Higher resolution sequence data.
UMI based DNA counting
Barcode based quantification with no PCR bias.
Easy to use
Get the kit
Next day shipping for the sample prep kit.
Upload results
Securely upload FASTQ results to the Loop's cloud pipeline.
Receive data
Download FASTQ and CSV files with classification and quantification data.
Outsource your sequencing
Process your samples in Loop's lab. Learn more about the service options.
Project highlight
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